- Winfried Denk – Max Planck Institute of Neurobiology (MPIN), Germany
- Fred Hamprecht – Interdisciplinary Center for Scientific Computing (IWR) and Department of Physics and Astronomy, Heidelberg Collaboratory for Image Processing (HCI), Germany
- Chris Guerin – VIB Ghent, Belgium
- Graham Knott – EPFL Lausanne, Switzerland
- Anna Kreshuk – IWR and Heidelberg Collaboratory for Image Processing (HCI), University of Heidelberg, Germany
- Peter O’Toole – University of York, United Kingdom
- Richard Webb – University of Queensland, Australia
Other confirmed speakers
- Theodore Alexandrov – EMBL Heidelberg, Germany
- Ilya Belevich – Institute of Biotechnology, University of Helsinki, Finland
- Silke Christiansen – HZB, Germany
- Lucy Collinson – Francis Crick Institute, United Kingdom
- Christel Genoud – Friedrich Miescher Institute for Biomedical Research, Switzerland
- Ben Giepmans – UMCG, The Netherlands
- Jan Harapin – University of Zurich, Switzerland
- Eija Jokitalo – University of Helsinki, Finland
- Matthia Karreman – EMBL Heidelberg, Germany
- Perrine Paul-Gilloteaux – INRIA, Rennes, France
- Roger Wepf – ETH Zurich, Switzerland
In Life Sciences, imaging in three dimensions is a prerequisite for the understanding of the fine organisation of cells and tissues. Whilst key biological functions are addressed by a large choice of fluorescence microscopy techniques, volume imaging by scanning electron microscopy offers now an unprecedented understanding of the ultrastructure of large biological objects. Correlating both imaging modalities, in 3D, is therefore a powerful way to link function to structure, even in complex biological systems.
This workshop will gather leading experts in the field of 3D correlative light and electron microscopy, with a particular focus on automated serial imaging by scanning electron microscopy. Two keynotes, from W. Denk and F. Hamprecht will cover the latest breakthroughs in automated serial imaging and 3D image analysis. The symposium will then alternate lectures and practical workshops to address a large spectrum of topics related to 3D CLEM, from the state of the art to recent biological applications.
Volume imaging and 3D correlative approaches are developing fast with increasing number of users and applications. This symposium aims not only at providing an update on the latest development in the field but also at connecting people. Experts and new-to-the-field will have the opportunity to share their experience and their views on the current methods and tools, but also exchange about the future challenges of these emerging techniques.
The session topics are as follows:
- 3D Correlative Light and Electron Microscopy
- The challenges of sample diversity
- From large volume imaging to data analysis and processing
- How 3D CLEM connects to other 3D Imaging Techniques
- Diversity of applications in biology
The workshops will include:
- Sample Preparation
- Array Tomography
- Image Processing
In addition there will be three panel discussions on broad topics within the scope of 3D Correlative Light and Electron Microscopy. This will be an opportunity to raise questions to experts in the field. We are encouraging participants to post questions on each of these topics before the event so that the session can cover the most pressing issues.
The topics will be:
- Large Data Handling
- Shared experiences in CLEM
- Facility Implementation – tips and tricks for implementing these techniques in a facility environment, marketing facility services, charging for services, equipment maintenance and consumables.
Participants will have the choice to attend all 6 workshops, or to substitute one or more workshops with panel discussions.This workshop will be followed by a Gatan 3view user group meeting.
- Yannick Schwab – EMBL Heidelberg, Germany
- Nicole Schieber – EMBL Heidelberg, Germany
- Robert Kirmse – Carl Zeiss Microscopy GmbH, Germany
- Hans-Jürgen Oberdiek – Carl Zeiss Microscopy GmbH, Germany
- Chris Guerin – VIB Ghent, Belgium
- Saskia Lippens – VIB Ghent, Belgium
- Conference Organiser: Diah Yulianti – EMBL Heidelberg, Germany